pLVX-XBP1 mNeonGreen NLS

LOCUS       pLVX-XBP1 mNeonGreen NLS                9066 bp ds-DNA     circular SYN 12-AUG-2021
DEFINITION  ER-stress sensor - XBP1 splicing fluorescent reporter with 
            mNeonGreen.
ACCESSION   .
VERSION     .
KEYWORDS    pLVX-XBP1 mNeonGreen NLS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9066)
  AUTHORS   Nougarede A, Tesniere C, Ylanko J, Rimokh R, Gillet G, Andrews DW
  TITLE     Improved IRE1 and PERK Pathway Sensors for Multiplex Endoplasmic 
            Reticulum Stress Assay Reveal Stress Response to Nuclear Dyes Used 
            for Image Segmentation.
  JOURNAL   Assay Drug Dev Technol. 2018 Aug 8. doi: 10.1089/adt.2018.862.
  PUBMED    30088945
REFERENCE   2  (bases 1 to 9066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9066
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    158..273
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        331..634
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        635..838
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     789..809
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     835..859
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             973..999
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1260..1964
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="VSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEE
                     LNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDG
                     ASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIST
                     FKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK
                     AFTDVMGMDELYK"
     regulatory      1512..1521
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1986..2012
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     promoter        2032..2531
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2067..2090)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2464..2483
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2552..3151
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2552..2571)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3048..3068
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3165..3753
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3218..3238)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3636..3647)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3960..4593
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4721..4737)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4721..4737)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4734..4756)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4745..4761
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4769..4799)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4814..4835
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4952..4969)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5123..5711)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5203..5222)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5882..6742)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6505..6524
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6743..6847)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    6895..7029
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6932..6951)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6986..7005
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7197..7830
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7877..8002
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8499..8732
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8917..8961
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
       61 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
      121 tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag gggggattgg
      181 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
      241 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
      301 tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa atggcccgcc
      361 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
      421 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
      481 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
      541 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
      601 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
      661 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
      721 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
      781 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
      841 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag
      901 acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag ctcaagcttc
      961 gaattcacca tgtaccccta cgacgtgccc gactacgccg gccttgtagt tgagaaccag
     1021 gagttaagac agcgcttggg gatggatgcc ctggttgctg aagaggaggc ggaagccaag
     1081 gggaatgaag tgaggccagt ggccgggtct gctgagtccg cagcactcag actacgtgca
     1141 cctctgcagc aggtgcaggc ccagttgtca cccctccaga acatctcccc ttggattctg
     1201 gcggtattga ctcttcagat tcagagtctg atatcctgtt gggcattcta ataggatccg
     1261 tgagcaaggg cgaggaggat aacatggcct ctctcccagc gacacatgag ttacacatct
     1321 ttggctccat caacggtgtg gactttgaca tggtgggtca gggcaccggc aatccaaatg
     1381 atggttatga ggagttaaac ctgaagtcca ccaagggtga cctccagttc tccccctgga
     1441 ttctggtccc tcatatcggg tatggcttcc atcagtacct gccctaccct gacgggatgt
     1501 cgcctttcca ggccgccatg gtagatggct ccggatacca agtccatcgc acaatgcagt
     1561 ttgaagatgg tgcctccctt actgttaact accgctacac ctacgaggga agccacatca
     1621 aaggagaggc ccaggtgaag gggactggtt tccctgctga cggtcctgtg atgaccaact
     1681 cgctgaccgc tgcggactgg tgcaggtcga agaagactta ccccaacgac aaaaccatca
     1741 tcagtacctt taagtggagt tacaccactg gaaatggcaa gcgctaccgg agcactgcgc
     1801 ggaccaccta cacctttgcc aagccaatgg cggctaacta tctgaagaac cagccgatgt
     1861 acgtgttccg taagacggag ctcaagcact ccaagaccga gctcaacttc aaggagtggc
     1921 aaaaggcctt taccgatgtg atgggcatgg acgagctgta caaggtcgac ggtggatcag
     1981 ggggacccgc cgccaagcgc gtgaagctgg actaatctag ataattctac cgggtagggg
     2041 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc
     2101 gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg
     2161 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc
     2221 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta
     2281 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg
     2341 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg
     2401 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc
     2461 cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc
     2521 cgggcctttc gacctgcagc ccaagcttac catgaccgag tacaagccca cggtgcgcct
     2581 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     2641 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     2701 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     2761 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     2821 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     2881 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     2941 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     3001 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     3061 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     3121 ctggtgcatg acccgcaagc ccggtgcctg accgcgtctg gaacaatcaa cctctggatt
     3181 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     3241 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     3301 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     3361 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     3421 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     3481 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     3541 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     3601 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     3661 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     3721 cgagtcggat ctccctttgg gccgcctccc cgcctggaat taattctgca gtcgagacct
     3781 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg
     3841 gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag
     3901 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa agaggggact
     3961 ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac
     4021 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
     4081 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
     4141 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     4201 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
     4261 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
     4321 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
     4381 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
     4441 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     4501 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     4561 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     4621 agtatttata acttgcaaag aaatgaatat cagagagtga gaggccttga cattgctagc
     4681 gtttaccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     4741 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     4801 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     4861 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     4921 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     4981 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     5041 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     5101 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     5161 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     5221 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     5281 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     5341 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     5401 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     5461 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     5521 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     5581 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     5641 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     5701 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     5761 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     5821 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     5881 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     5941 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     6001 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     6061 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     6121 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     6181 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     6241 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     6301 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     6361 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     6421 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     6481 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     6541 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     6601 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     6661 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     6721 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     6781 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     6841 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatcaacttg
     6901 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     6961 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     7021 gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac
     7081 cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa
     7141 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga
     7201 agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca
     7261 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
     7321 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
     7381 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
     7441 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
     7501 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg
     7561 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
     7621 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct
     7681 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     7741 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     7801 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     7861 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     7921 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     7981 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     8041 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     8101 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     8161 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     8221 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     8281 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     8341 ggccggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     8401 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     8461 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     8521 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     8581 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     8641 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     8701 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     8761 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     8821 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     8881 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     8941 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     9001 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     9061 tctata
//